Using CRISPR/Cas9 to Edit Genes in Soybean

Transgenic soybean plants with mutations in up to three target genes have been successfully created using CRISPR/Cas9 technology.

• CRISPR/Cas9 technology allows for targeted genome editing in various plant species, including soybean.
• Two platforms have been developed for cloning both single and multiple CRISPR targets in soybean.
• Reagent delivery methods are described to enhance the efficiency of CRISPR editing in soybean hairy roots and regenerated plants.
• The protocols address key challenges that can hinder successful gene editing in soybean.

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