Genome-wide CRISPR screening found a non-neuron receptor called hemojuvelin for botulinum toxins A and C

Knockout of hemojuvelin (HJV) in mice markedly reduces toxin accumulation at the muscle surface.

• Hemojuvelin (HJV) is identified as a non-neuronal receptor for botulinum neurotoxins A and C.
• HJV interacts directly with the toxin's receptor-binding domain, facilitating toxin binding.
• Loss of HJV significantly decreases sensitivity to botulinum neurotoxins, leading to reduced paralysis and lethality.
• HJV is crucial for toxin accumulation at neuromuscular junctions, which is essential for subsequent internalization by neuronal receptors.
• These findings enhance understanding of how botulinum neurotoxins enter cells and suggest potential strategies for addressing toxin exposure.

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