We can’t show the full text here under this license. Use the link below to read it at the source.
Genome‐wide analyses of PAM‐relaxed Cas9 genome editors reveal substantial off‐target effects by ABE8e in rice
Genome-wide study shows ABE8e gene editor causes many off-target changes in rice
AI simplified
Abstract
ABE8e results in ~500 genome-wide A-to-G off-target mutations at TA motif sites per transgenic plant.
- Genome-wide of various Cas9 nucleases and base editors in rice have been examined.
- Distinct off-target sites are preferred by Cas9 nucleases and base editors when using the same guide RNA.
- SpRY editors generate additional, but minor, off-target mutations from newly created guide RNAs.
- The preference of ABE8e for the TA motif is evident at both off-target and target sites.
- from tissue culture significantly contributes to background mutations in edited plants.
AI simplified
Key numbers
~500
Off-target A-to-G mutations per plant
Average number of A-to-G mutations induced by ABE8e in transgenic rice plants.