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Precise DNA base editing with low unwanted changes in mammal embryos using glycosylase enzymes
Updated
Abstract
Essence
AYBE and gGBE edited mammalian embryos efficiently with rare DNA off-target signals, though AYBE-derived TadA8e-V106W showed low-frequency RNA off-target editing.
Evidence
Platform experiments used GOTI in mouse embryos, transcriptome-wide RNA analysis, and editing tests in mouse and sheep embryos plus newborn lambs.
Caveat
The abstract reports embryo and livestock editing performance, not clinical safety or long-term outcomes after editing.
Simplified
Key numbers
47.37% to 99.90%
A-to-other Editing Efficiency
Editing efficiencies for AYBE-V106W across various target sites.
21.00% to 85.29%
G-to-other Editing Efficiency
Editing efficiencies for gGBE in sheep embryos.