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HIV-1 NC-induced stress granule assembly and translation arrest are inhibited by the dsRNA binding protein Staufen1
The double-stranded RNA binding protein Staufen1 blocks HIV-1 NC from causing stress granules and stopping protein production
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Abstract
Overexpression of the nucleocapsid (NC) protein leads to significant reductions in virus production and host cell mRNA translation.
- NC induces the assembly of stress granules (SG) in host cells, which is resistant to blockade by the HIV-1 capsid (CA).
- The reduction in host cell mRNA translation was confirmed through assays measuring newly synthesized proteins.
- Coexpression of Staufen1 completely reversed the inhibition of NC-mediated SG assembly and restored global mRNA translation and virus production.
- NC expression resulted in the phosphorylation of protein kinase R (PKR) and eIF2α, which was also inhibited by Staufen1 coexpression.
- The findings highlight a novel role of NC in modulating the host stress response during HIV-1 replication.
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