Hydrogen peroxide modulates clock gene expression via PRX2-STAT3-REV-ERBα/β pathway

Hydrogen peroxide upregulated the protein expression of circadian genes in NIH3T3 cells.

• Circadian genes such as Clock, Bmal1, Per1/2, and Cry1/2 are influenced by oxidative stress.
• External factors, known as zeitgebers, can regulate biological rhythms through circadian genes.
• Hydrogen peroxide may alter the circadian rhythm of Bmal1-luciferase via specific proteins RORα, REV-ERBα, and REV-ERBβ.
• The regulation of biological rhythm by hydrogen peroxide is associated with the PRX2-STAT3-REV-ERBα/β pathway.
• These findings suggest a mechanism where non-transcriptional processes interact with transcriptional feedback loops.

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