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Abstract
An optimized Fanzor2 system achieves robust editing at challenging genomic sites in plants.
- Native Fanzor2 nucleases show limited editing capability in plant genomes.
- Engineering modifications, including RNA optimization and protein mutagenesis, enhance editing performance.
- The optimized system enables cytosine base editing, expanding the potential for precise genetic changes.
- Versatile crop genome modification can be achieved through transformation or viral delivery methods.
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