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Comparison of ionizable lipids for lipid nanoparticle mediated DNA delivery
Comparing charged fats for delivering DNA using lipid nanoparticles
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Abstract
Efficient transgene expression was achieved with a minimum amount of 1 pg DNA per initially plated cells.
- Lipid nanoparticles (LNPs) are effective for RNA-based gene delivery and may also be promising for DNA delivery in gene replacement therapies.
- The study evaluated different ionizable lipids (DLin-MC3-DMA, SM-102, and ALC-0315) for their impact on DNA expression plasmid delivery.
- At an optimized N/P ratio of 6, DNA-LNPs exhibited small, spherical, and monodisperse characteristics with anionic ζ-potential.
- Zebrafish studies indicated that selected DNA-LNPs achieved prolonged circulation in blood and reduced clearance by macrophages.
- In wildtype mice, DNA-LNPs incorporating ALC-0315 showed superior transfection efficiency compared to other lipid types.
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