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Abstract
IscB proteins may use a single noncoding RNA for RNA-guided cleavage of double-stranded DNA.
- IscB is a putative nuclease related to the ancestors of the RNA-guided endonuclease Cas9.
- Evolutionary analysis and RNA sequencing were utilized to understand the evolution of CRISPR-Cas9 systems from IS200/IS605 transposons.
- IscB can be harnessed for genome editing in human cells.
- TnpB, another protein from the IS200/IS605 family, exhibits RNA-guided nuclease activity and is likely an ancestor of Cas12 endonucleases.
- A new class of transposon-encoded RNA-guided nucleases, named OMEGA, is proposed.
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