JianPi-QingHua formula attenuates nonalcoholic fatty liver disease by regulating the AMPK/SIRT1/NF-κB pathway in high-fat-diet-fed C57BL/6 mice

Apr 11, 2023Pharmaceutical biology

JianPi-QingHua formula reduces fatty liver disease by affecting energy and inflammation pathways in high-fat diet mice

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Abstract

JianPi-QingHua formula (JPQH) reduced body weight, liver weight, and levels of serum total cholesterol and triglycerides in mice with high-fat diet-induced .

JPQH treatment significantly reduced epididymal fat mass and blood glucose levels compared to the high-fat diet group.
Serum levels of alanine aminotransferase and aspartate aminotransferase were markedly decreased with JPQH intervention.
Histopathological analysis revealed that JPQH improved liver conditions, suppressing lipid accumulation and inflammation.
JPQH may enhance liver health by activating SIRT1/AMPK signaling while inhibiting the NF-κB pathway.

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CONTEXT: (NAFLD) is a common liver disease, accompanied by liver lipid accumulation and inflammation. JianPi-QingHua formula (JPQH), a Chinese herbal formula, exhibits effects on obesity and T2DM. However, the hepatoprotective effect of JPQH has not been elucidated.

OBJECTIVE: To investigate the hepatoprotective effect of JPQH in NAFLD induced by a high-fat diet (HFD) in mice.

MATERIALS AND METHODS: C57BL/6J mice were divided into four groups and fed a normal-fat diet (ND), high-fat diet (HFD), HFD + JPQH (2.5 g/kg), or HFD + metformin (300 mg/kg) for 6 weeks, respectively. Furthermore, the body weight, epididymal fat mass, blood glucose, and liver weight were measured. Serum total cholesterol (TC), triglycerides (TG), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were performed. Hematoxylin and eosin staining and Oil Red O staining were observed in hepatic histopathological changes. Western blotting and quantitative real-time polymerase chain reaction were utilized to assess the key protein expression of hepatic lipid metabolism and inflammation.

RESULTS: Compared with the HFD group, JPQH could reduce body weight, epididymal fat mass, blood glucose and liver weight ( < 0.05), and markedly decreased the levels of serum TC, TG, ALT, AST ( < 0.05). Additionally, JPQH improved liver pathological changes. Consistent with the hepatic histological analysis, JPQH intervention suppressed lipid accumulation and inflammatory responses. Mechanistically, JPQH boosted SIRT1/AMPK signalling, and attenuated NF-κB pathway, which suppressed inflammatory responses. p p

DISCUSSION AND CONCLUSIONS: These findings indicate that JPQH supplementation protected against HFD-induced NAFLD by regulating SIRT1/AMPK/NF-κB pathway, which provides a theoretical basis for the clinical treatment of patients with NAFLD.

Key numbers

n=10

Body Weight Decrease

Body weight of HFD-fed mice after 6 weeks of JPQH treatment.

n=6

Serum Cholesterol Reduction

Levels of serum total cholesterol after JPQH intervention.

n=6

Liver Weight Reduction

Liver weights of HFD-fed mice after JPQH treatment.

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JianPi-QingHua formula attenuates nonalcoholic fatty liver disease by regulating the AMPK/SIRT1/NF-κB pathway in high-fat-diet-fed C57BL/6 mice

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