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Detection and Identification of Kratom (Mitragyna speciosa) and Marijuana (Cannabis sativa) by a Real‐Time Polymerase Chain Reaction High‐Resolution Melt Duplex Assay,
Detecting and Identifying Kratom and Marijuana Using a Fast DNA Testing Method
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Abstract
The SLS2 primers identified Mitragyna speciosa DNA at 0.05 ng/μL.
- Mitragyna speciosa, known as kratom, is used as an alternative for pain relief.
- Melt temperatures for the SGD and SLS2 genes were found to be 77.08 ± 0.38°C and 77.61 ± 0.46°C, respectively, using SYBRGreen I.
- Using RadiantGreen, the melt temperatures for SGD and SLS2 were 80.18 ± 0.27°C and 80.59 ± 0.08°C, respectively.
- With LCGreenPLUS, the observed melt temperatures for SGD and SLS2 were 82.19 ± 0.04°C and 82.62 ± 0.13°C, respectively.
- A duplex reaction successfully differentiated MS and Cannabis sativa by melt peaks of 82.63 ± 0.35°C and 85.58 ± 0.23°C, respectively.
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