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Detection and Identification of Psilocybe cubensis DNA Using a Real‐Time Polymerase Chain Reaction High Resolution Melt Assay,
Detecting and Identifying Psilocybe cubensis DNA Using a Sensitive DNA Test
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Abstract
The observed high resolution melt (HRM) temperatures for primer sets targeting Psilocybe DNA were 78.85 ± 0.31°C and 73.22 ± 0.61°C using SYBRGreen I dye.
- Two primer sets were designed to specifically target the DNA of Psilocybe cubensis.
- Eighteen samples, including twelve different mushroom species, were analyzed for DNA extraction.
- PCR was performed using various intercalating dyes to assess DNA amplification.
- High resolution melt temperatures were measured to evaluate the performance of the primers.
- The primer sets demonstrated reproducibility and sensitivity in the amplification process.
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