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Abstract
Nano-flow cytometry provides a robust method for sizing lipid nanoparticles (LNPs) with high concordance to cryogenic transmission electron microscopy.
- Lipid nanoparticles are designed to deliver therapeutic substances like mRNA effectively.
- Key characteristics for evaluating LNP formulations include size distribution and loading efficiency.
- Various techniques exist for measuring nanoparticle size, each with distinct advantages and limitations.
- The study compared four sizing methods: nanoparticle tracking analysis, dynamic light scattering, two nano cytometers, and cryogenic transmission electron microscopy.
- Results indicated that measurements from nano-flow cytometry closely matched those from cryogenic transmission electron microscopy.
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