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Abstract
9G8-attached PMSEA mRNA-LNPs showed significantly enhanced cell association and transfection efficiency with an EGFR-positive cell line.
- Current immunotherapeutic mRNA approaches lack site- and immune-cell-specific delivery, leading to off-target immune responses.
- Commercial lipid nanoparticle formulations tend to accumulate significantly in the liver during clearance.
- Bioconjugation methods were explored to attach targeting antibodies to polymer-functionalized mRNA lipid nanoparticles.
- The incorporation of cyclooctene functionality in PMSEA-DSPE allowed for effective conjugation to the tetrazine-functionalized nanobody 9G8.
- Active targeting through 9G8 conjugation could enhance mRNA-LNP platform efficacy.
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