Complete sequence of Litopenaeus vannamei (Crustacea: Decapoda) vitellogenin cDNA and its expression in endocrinologically induced sub-adult females

Aug 27, 2005General and comparative endocrinology

Complete genetic sequence of a key egg protein and its production in hormone-treated young female shrimp

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Abstract

The full-length cDNA of the vitellogenin gene from Pacific white shrimp Litopenaeus vannamei encodes a 2587 amino acid polypeptide with up to 85% identity to vitellogenin from other penaeid species.

The vitellogenin gene is expressed during the development of eggs in decapod crustaceans.
Peptide mass fingerprints indicate that a specific cleavage site in the vitellogenin polypeptide likely undergoes cleavage.
Five high-density lipoprotein polypeptides were isolated from the vitellogenic ovary, and their masses were determined.
Periodic ovarian growth and resorption were observed during multiple molt cycles in sub-adult females.
The expression of vitellogenin in the ovaries was significantly higher in induced females compared to the hepatopancreas at all stages of the molt cycle.

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The gene that encodes vitellogenin (Vg), the precursor of the major yolk protein, vitellin, is expressed during vitellogenesis in decapod crustaceans. In this study, we sequenced the full-length cDNA from the Pacific white shrimp Litopenaeus vannamei Vg gene (LvVg). This is the first open thelycum penaeid shrimp Vg cDNA to be sequenced. The transcript encodes a 2587 amino acid polypeptide with up to 85% identity to Vg of different penaeid species. Peptide mass fingerprints (PMFs) of the vitelline polypeptides suggest that the predicted endoprotease cleavage site at amino acids 725-728 does indeed undergo cleavage. Five prominent high-density lipoprotein polypeptides of masses 179, 113, 78, 61, and 42kDa were isolated from vitellogenic ovary, and their PMFs were determined by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) spectrometry. It is likely that these polypeptides are all products of the LvVg gene. Removal of the X-organ sinus gland complex (XO-SG), which secretes the neurohormones that control the endocrine system regulating molt and reproduction, can induce both these processes. During the course of a number of molt cycles in induced sub-adult females, periodic ovarian growth and resorption were observed. Ovary growth correlated with LvVg expression in both the hepatopancreas and the ovary. Expression in ovaries of induced intermolt-early premolt females was significantly higher compared to all other sub-groups. Expression in ovaries of induced females was significantly higher compared to hepatopancreas at all molt cycle stages. Periodicity of molt and vitellogenesis in endocrinologically induced sub-adult shrimps may serve as a model to study alternate regulation of gene expression during these two processes.

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Complete sequence of Litopenaeus vannamei (Crustacea: Decapoda) vitellogenin cDNA and its expression in endocrinologically induced sub-adult females

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