Full text is available at the source.
Abstract
A linear detection range of 10 fM-1 nM with a limit of detection of 2 fM was achieved for Candidatus Liberibacter asiaticus gene fragments.
- The biosensor integrates rolling circle amplification and CRISPR/Cas12a activities for enhanced detection.
- Target-initiated amplification leads to the degradation of specific DNA, restoring electrochemiluminescence signals.
- High specificity was demonstrated for detecting the ribonucleotide-diphosphate reductase subunit beta gene fragments.
- The system eliminates the need for multiple incubation steps, streamlining the detection process.
- Satisfactory performance was observed in detecting HLB pathogens in citrus leaf samples.
Simplified