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Mast Cell-Dependent Allergic Responses Are Inhibited by Ethanolic Extract of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) Testa
Ethanol extract of adlay seed coat reduces allergic reactions involving mast cells
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Abstract
The ethyl acetate fraction from adlay testa suppressed degranulation in rat basophilic leukemia (RBL)-2H3 cells stimulated by 1 microM A23187.
- Adlay testa exhibited potent inhibitory activity against beta-hexosaminidase release, a marker of mast cell degranulation.
- The 20%-80% EtOAc/Hex subfractions significantly inhibited histamine release with an IC(50) of 75-100 microg/mL.
- Adlay testa also suppressed the secretion of interleukin (IL)-4, IL-6, and tumor necrosis factor-alpha in RBL-2H3 cells.
- Inhibition of degranulation was associated with reduced phosphorylation of ERK, a key signaling molecule.
- 4-hydroxyacetophenone and p-coumaric acid were identified as the two major active compounds in the adlay testa subfractions.
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