METTL3/IGF2BP1 influences the development of non‐small‐cell lung cancer by mediating m6A methylation modification of TRPV1

Aug 1, 2024Thoracic cancer

How METTL3 and IGF2BP1 may affect lung cancer growth by changing chemical marks on TRPV1

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Abstract

expression was upregulated in non-small-cell lung cancer (NSCLC) and associated with poor prognosis.

Silencing TRPV1 inhibited NSCLC cell growth and metastasis while inducing apoptosis.
Reduced TRPV1 expression also repressed the polarization of M2 macrophages in the tumor environment.
METTL3 and IGF2BP1 were found to regulate TRPV1 expression through a specific chemical modification process.
Deficiency of METTL3 led to reduced growth and spread of NSCLC cells and increased apoptosis.
Overexpression of TRPV1 counteracted the effects of METTL3 deficiency on NSCLC cell behavior.
In a murine model, METTL3 knockdown suppressed tumor growth by regulating TRPV1 expression.

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BACKGROUND: Methyltransferase 3 (METTL3) accelerates N6-methyladenosine (m6A) modifications and affects cancer progression, including non-small-cell lung cancer (NSCLC). In this study, we aimed to explore the regulatory mechanisms of METTL3 underling NSCLC.

METHODS: Immunohistochemical assay, quantitative real-time polymerase chain reaction (qRT-PCR) assay, and western blot assay were conducted for gene expression. MTT assay and colony formation assay were performed to explore cell proliferation capacity. Cell apoptosis and THP-1 cell polarization were estimated by flow cytometry analysis. Cell migration and invasion capacities were evaluated by transwell assay. Methylated RNA immunoprecipitation assay, dual-luciferase reporter assay, actinomycin D treatment and RIP assay were performed to analyze the relationships of METTL3, insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1), and transient receptor potential cation channel subfamily V member 1 (). The functions of METTL3 and TRPV1 in vivo were investigated through establishing the murine xenograft model.

RESULTS: TRPV1 expression was upregulated in NSCLC and related poor prognosis. TRPV1 silencing inhibited NSCLC cell growth and metastasis, induced NSCLC cell apoptosis, and repressed M2 macrophage polarization. The results showed that METTL3 and IGF2BP1 could regulate TRPV1 expression through modification. Moreover, METTL3 deficiency inhibited NSCLC cell growth, metastasis, and M2 macrophage polarization and facilitated NSCLC cell apoptosis, while TRPV1 overexpression restored the impacts. In addition, METTL3 knockdown restrained tumor growth in vivo via regulating TRPV1 expression.

CONCLUSION: METTL3 bound to IGF2BP1 and enhanced IGF2BP1's m6A recognition of TRPV1 mRNA, thereby promoting NSCLC cell growth and metastasis, and inhibiting M2 macrophage polarization.

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Patient Cohort Size

Clinical specimens collected from NSCLC patients.

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Expression Impact

High expression group vs. low expression group.

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METTL3/IGF2BP1 influences the development of non‐small‐cell lung cancer by mediating m6A methylation modification of TRPV1

Abstract

Key numbers

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