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Modulation of the circadian clock by glucocorticoid receptor isoforms in the H295R cell line
How Different Forms of Stress Hormone Receptors Affect the Body’s Internal Clock in Human Adrenal Cells
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Abstract
Rhythmic expression of clock genes was observed in the human adrenocortical cell line H295R.
- Synchronization with serum shock confirmed rhythmic oscillation of clock genes PER1, PER2, REV-ERBα, and ARNTL.
- H295R cells exhibited rhythmic expression of PER1, PER2, CRY1, and ARNTL even without serum shock.
- Glucocorticoid treatment induced rapid changes in PER1, PER2, and CRY1 levels in a GRα-dependent manner.
- Continuous glucocorticoid stimulation after 6 hours suppressed REV-ERBα expression.
- Administration of a glucocorticoid receptor antagonist disrupted the circadian oscillation of clock genes.
- Overexpression of the GRß isoform did not change clock gene expression but prevented GRα-related suppression of REV-ERBα.
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