Mechanism of Mongolian medical warm acupuncture in treating insomnia by regulating miR-101a in rats with insomnia

Jul 5, 2017Experimental and therapeutic medicine

How Mongolian warm acupuncture may help insomnia by changing a specific gene regulator in rats

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Abstract

Mongolian medicine warm acupuncture resulted in 141 differentially expressed microRNAs in the brain tissue of insomnia rat models.

The expression level of miR-101a in the hippocampus of insomnia rats increased significantly 72 hours after treatment with miR-101a mimics (P<0.05).
Neuronal cell activity increased significantly in insomnia rats transfected with miR-101a inhibitors at 72 hours (P<0.05).
The PAX8 protein expression in neuronal cells of insomnia model rats was significantly inhibited after treatment with miR-101a mimics (P<0.01).
Levels of interleukins IL-1, IL-2, and IL-6, along with tumor necrosis factor-α, decreased significantly in various brain regions following warm acupuncture (P<0.05).
Noradrenaline, dopamine, and glutamic acid levels significantly decreased after warm acupuncture or western medicine treatment (P<0.05).

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MicroRNAs (miRNAs or miRs) and the target genes before and after warm acupuncture at the genetic level were assessed, and the cytokines and neurotransmitters related to insomnia were studied. Male Sprague-Dawley rats were used to create PCPA insomnia rat models and randomly divided into the normal, model, warm acupuncture, and drug groups. The Dinghui Acupoint, Heyi Acupoint, and Xin Acupoint were inserted in the Mongolian medicine warm acupuncture group. The differential expression profile of microRNA in the brain tissue of the insomnia rats was determined before and after Mongolian medicine warm acupuncture for establishment of miR-101a mimics and inhibitor. qPCR was used to detect the expression level of miR-101a. Western blotting was used to detect the expression level of PAX8. The rats receiving Mongolian medicine warm acupuncture had 141 miRNAs with differential expression compared with the normal rats. The expression level of miR-101a in the cells of the hippocampus of the insomnia rats transfected with miR-101a mimics increased significantly at 72 h (P<0.05). The activity of the neuronal cells transfected with miR-101a inhibitor increased significantly at 72 h (P<0.05). The western blotting result indicated that the expression of the PAX8 protein in the neuronal cells of the insomnia model rats was inhibited and downregulated significantly at 72 h after addition of miR-101a mimics compared with that in the scramble added group (P<0.01). The levels of the interleukins IL-1, IL-2, and IL-6 and the tumor necrosis factor-α in the hypothalamus, hippocampus, and prefrontal cortex decreased significantly compared with those in the blank control group (P<0.05). The levels of noradrenaline, dopamine, and glutamic decreased significantly following warm acupuncture or western medicine treatment (P<0.05). In conclusion, this study demonstrates that the upregulation of miR-101a in the rats treated with warm acupuncture is directly associated with PAX8 regulation.

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Mechanism of Mongolian medical warm acupuncture in treating insomnia by regulating miR-101a in rats with insomnia

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