Highly sensitive monkeypox virus detection using boosted CRISPR signals on a DNA-based sensor

An electrochemical biosensor can detect monkeypox virus (MPXV) in 10% human saliva.

• The biosensor integrates a CRISPR/Cas12a signal amplification strategy with a tetrahedral DNA nanostructure for enhanced sensitivity.
• MPXV DNA triggers the cleavage activity of the Cas12a protein, facilitating a hybridization chain reaction on the sensing interface.
• The resulting horseradish peroxidase-labeled product generates an amplified electrochemical signal through a specific reaction.
• The biosensor demonstrates a wide linear range, an ultralow detection limit, and high selectivity against non-target viruses.
• It shows high reproducibility and stability, making it suitable for point-of-care testing applications.

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