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Abstract
A cost-effective microfluidic setup for synthesizing mRNA lipid nanoparticles (LNPs) was developed.
- LNPs synthesized with the custom microfluidic device exhibit uniform structure and dense cores.
- Ethanol injection leads to broader size distributions and a more inhomogeneous internal structure in LNPs.
- Small angle X-ray scattering data suggest tighter interactions between mRNA and lipids in LNPs from the custom device compared to those from a commercial device.
- Polysarcosine (pSar)-modified LNPs demonstrate higher transfection efficiency associated with increased surface roughness compared to PEGylated LNPs.
- The manufacturing approach has a minor influence on mRNA expression in vivo.
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