New combined test for on-site detection of high-risk HPV types

An integrated platform enables 100% concordance with qPCR for detecting eight key high-risk HPV genotypes in under 50 minutes.

• The platform combines multiplex recombinase polymerase amplification with CRISPR-Cas12a for sensitive detection.
• Single-copy sensitivity is achieved within 50 minutes at 37 °C, overcoming limitations of traditional methods.
• Clinical validation identified three additional low viral-load positives that conventional methods missed.
• The system allows for visual readout under UV light and does not require specialized equipment.
• This approach could facilitate accessible molecular diagnostics in resource-limited settings.

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