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Muscle-targeted nanoparticle vaccines trigger strong immune responses against chickenpox virus
Updated
Abstract
A library of 21 novel ionizable lipids was synthesized to optimize mRNA delivery, resulting in high transfection efficiency in muscle tissue with reduced gene expression in the liver.
- Three LNPs, YK-305, YK-310, and YK-319, were identified as having high efficiency for mRNA delivery.
- The LNP formulations demonstrated favorable properties with low cytotoxicity.
- Intramuscular injection of firefly luciferase mRNA-LNPs achieved significant transfection in muscle tissue.
- Encapsulation of varicella zoster virus glycoprotein E mRNA led to robust immune responses specific to the gE antigen.
- The novel LNP formulations showed improved selectivity and immunogenicity compared to the ionizable lipid SM-102 used in Moderna's Spikevax.
- Safety of the gE-mRNA LNP formulations was confirmed in a mouse model.
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