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New CRISPR/Cas12a method for detecting genetic material in biosensors

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated

Abstract

Non-canonical CRISPR/Cas12a-based biosensors may improve nucleic acid detection capabilities.

  • Canonical CRISPR/Cas12a biosensors are known for their high specificity and simple operation but struggle with sensitivity and efficiency.
  • Non-canonical CRISPR/Cas12a biosensors could address limitations related to target scope, multiplexing, and signal detection.
  • This review summarizes the applications, principles, and characteristics of non-canonical CRISPR/Cas12a-based biosensors.
  • Performance metrics and future prospects of these advanced biosensors are discussed.

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Funding

Competing interests

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
PubMed

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