Non-canonical CRISPR/Cas12a-based technology: A novel horizon for biosensing in nucleic acid detection

Jan 17, 2024Talanta

New CRISPR/Cas12a method for detecting genetic material in biosensors

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Abstract

Non-canonical CRISPR/Cas12a-based biosensors may improve nucleic acid detection capabilities.

Canonical CRISPR/Cas12a biosensors are known for their high specificity and simple operation but struggle with sensitivity and efficiency.
Non-canonical CRISPR/Cas12a biosensors could address limitations related to target scope, multiplexing, and signal detection.
This review summarizes the applications, principles, and characteristics of non-canonical CRISPR/Cas12a-based biosensors.
Performance metrics and future prospects of these advanced biosensors are discussed.

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Nucleic acids are essential biomarkers in molecular diagnostics. The CRISPR/Cas system has been widely used for nucleic acid detection. Moreover, canonical CRISPR/Cas12a based biosensors can specifically recognize and cleave target DNA, as well as single-strand DNA serving as reporter probe, which have become a super star in recent years in the field of nucleic acid detection due to its high specificity, universal programmability and simple operation. However, canonical CRISPR/Cas12a based biosensors are hard to meet the requirements of higher sensitivity, higher specificity, higher efficiency, larger target scope, easier operation, multiplexing, low cost and diversified signal reading. Then, advanced non-canonical CRISPR/Cas12a based biosensors emerge. In this review, applications of non-canonical CRISPR/Cas12a-based biosensors in nucleic acid detection are summarized. And the principles, peculiarities, performances and perspectives of these non-canonical CRISPR/Cas12a based biosensors are also discussed.

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Non-canonical CRISPR/Cas12a-based technology: A novel horizon for biosensing in nucleic acid detection

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