Full text is available at the source.
Abstract
The ENCODE4 Functional Characterization Centers conducted 108 screens in human cell lines, comprising >540,000 perturbations across 24.85 megabases of the genome.
- Pooled, noncoding CRISPR screens may enhance the study of gene regulatory mechanisms.
- Guidelines were established for screening noncoding elements with CRISPR interference, focusing on detecting CREs with variable transcriptional effects.
- CASA was identified as the analysis tool that produces the most conservative calls for CREs and is resilient to low-specificity single guide RNA artifacts.
- A subtle DNA strand bias was observed for CRISPRi in transcribed regions, which may impact screen design and analysis.
- An accessible data resource with predesigned single guide RNAs is available for targeting 3,275,697 ENCODE SCREEN candidate CREs.
Simplified