Norepinephrine causes nighttime eye pressure increase in mice through the RHOB protein

Eighteen genes, including RHOB, were identified as being upregulated by norepinephrine in human trabecular meshwork cells.

• Intraocular pressure (IOP) increases at night, influenced by norepinephrine from the superior cervical ganglion.
• The regulation of IOP rhythm is linked to the dynamics of aqueous humor outflow through the trabecular meshwork.
• RHOB deficiency appears to enhance the ability of trabecular meshwork cells to clear debris and counteract norepinephrine's effects on permeability.
• Inhibitors of RHO and ROCK reduced nocturnal and norepinephrine-induced increases in IOP in mice.
• These findings indicate that RHOB may play a crucial role in regulating IOP rhythm and could be a target for glaucoma therapies.

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