One-Pot Isothermal Linear Amplification and Cas12a-Based Nucleic Acid Detection

CATNAP can detect HPV-16 in crude cell lysates of cervical cancer cells using minimal equipment.

• CATNAP integrates isothermal DNA amplification with Cas12a detection in a single reaction.
• The method utilizes a nicking enzyme and DNA polymerase to continuously generate single-stranded DNA.
• High catalytic efficiency was achieved through optimization of enzyme combinations and buffer conditions.
• CATNAP successfully differentiates between high- and low-risk HPV strains.
• This approach may improve accessibility for accurate disease detection in resource-limited settings.

AI simplified