Ordered changes in histone modifications at the core of the Arabidopsis circadian clock

Dec 14, 2012Proceedings of the National Academy of Sciences of the United States of America

Step-by-step changes in gene packaging at the center of the Arabidopsis daily biological clock

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Abstract

Chromatin remodeling plays a critical role in the circadian clock function of Arabidopsis thaliana.

  • The circadian oscillation is associated with the sequential accumulation of specific chemical modifications on histones, including H3 acetylation and H3K4 trimethylation.
  • Inhibition of histone acetylation and H3K4 trimethylation disrupts the expression of clock-related genes.
  • Blocking H3K4 trimethylation results in increased binding of clock repressors, indicating its role in the transition from gene activation to repression.
  • Misexpression of the histone methyltransferase SET DOMAIN GROUP 2/ARABIDOPSIS TRITHORAX RELATED 3 alters oscillator gene expression and histone modifications.
  • A chromatin-dependent mechanism of clock gene activation may be conserved across both plant and mammal circadian systems.

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