Full text is available at the source.
Rapid detection of rice diseases using a CRISPR-based DNA test without PAM requirement
Updated
Abstract
The Cas-PfLAMP assay detects rice pathogens with high sensitivity down to 3 copies.
- The assay identifies three specific rice pathogens: Xanthomonas oryzae pv. Oryzae, rice stripe virus, and rice black-streaked dwarf virus.
- High specificity is achieved through dual recognition mechanisms involving primer sets and CRISPR/Cas12a components.
- Results indicate a rapid detection capability, requiring approximately 50 minutes for on-site testing.
- Field sample analysis demonstrates the effective performance of the Cas-PfLAMP platform.
- This method may be adaptable for diagnosing other plant diseases and conducting nucleic acid tests in various field settings.
Simplified