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Abstract
Lipid nanoparticles functionalized with the RVG29 peptide significantly improve neuronal transfection in the mouse brain.
- Systemic delivery of mRNA to the brain is hindered by the blood-brain barrier and liver accumulation of delivery vehicles.
- Peptide-functionalized lipid nanoparticles were designed to target receptors on brain endothelial cells and neurons.
- Click chemistry was used to attach specific peptides (RVG29, T7, AP2, mApoE) to the lipid nanoparticles.
- Enhanced mRNA transfection was observed in the mouse brain with RVG29-functionalized nanoparticles.
- Functionalization with peptides reduced the delivery of mRNA to the liver after systemic administration.
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