PhERF6, interacting with EOBI, negatively regulates fragrance biosynthesis in petunia flowers

Jul 5, 2017The New phytologist

PhERF6 and EOBI work together to reduce fragrance production in petunia flowers

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Abstract

Silencing PhERF6 resulted in a significant increase in volatile concentrations emitted by petunia flowers.

PhERF6 was identified as a negative regulator of volatile benzenoid biosynthesis in petunia flowers.
Expression of PhERF6 is temporally and spatially linked to floral scent production and is upregulated by ethylene.
Modifying the mRNA levels of PhERF6 influenced the expression of floral scent-related genes, including ODO1.
PhERF6 interacts with the EOBI protein, which has two DNA binding domains, suggesting a competitive regulatory mechanism.

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In petunia, the production of volatile benzenoids/phenylpropanoids determines floral aroma, highly regulated by development, rhythm and ethylene. Previous studies identified several R2R3-type MYB trans-factors as positive regulators of scent biosynthesis in petunia flowers. Ethylene response factors (ERFs) have been shown to take part in the signal transduction of hormones, and regulation of metabolism and development processes in various plant species. Using virus-induced gene silencing technology, a negative regulator of volatile benzenoid biosynthesis, PhERF6, was identified by a screen for regulators of the expression of genes related to scent production. PhERF6 expression was temporally and spatially connected with scent production and was upregulated by exogenous ethylene. Up-/downregulation of the mRNA level of PhERF6 affected the expression of ODO1 and several floral scent-related genes. PhERF6 silencing led to a significant increase in the concentrations of volatiles emitted by flowers. Yeast two-hybrid, bimolecular fluorescence complementation and co-immunoprecipitation assays indicated that PhERF6 interacted with the N-terminus of EOBI, which includes two DNA binding domains. Our results show that PhERF6 negatively regulates volatile production in petunia flowers by competing for the binding of the c-myb domains of the EOBI protein with the promoters of genes related to floral scent.

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PhERF6, interacting with EOBI, negatively regulates fragrance biosynthesis in petunia flowers

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