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PhieABEs: a PAM‐less/free high‐efficiency adenine base editor toolbox with wide target scope in plants
PhieABEs: A highly efficient adenine base editing tool without PAM limits for targeting many sites in plants
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Abstract
PhieABEs demonstrate nearly 100% editing efficiency at some tested sites in rice.
- PhieABEs are a new toolbox combining an evolved adenosine deaminase and a DNA-binding domain that enhances editing capabilities.
- This toolbox significantly improves base-editing activity and expands the target range compared to existing systems.
- The hyper ABE8e-DBD-SpRYn variant showed a high proportion of homozygous base substitutions within the editing windows.
- No off-target changes were observed with the hyper ABE8e-DBD-SpRYn variant under the tested conditions.
- The original single guide RNA was found to be more compatible with PhieABEs than the evolved version.
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Key numbers
100%
Editing Efficiency
Editing efficiency at select target sites with .
78.5%
Average Editing Efficiency
Average editing efficiency of hyABE8e-SpRY across tested sites.