Phosphorylation of REV-ERB proteins' front regions controls their location inside cells

Phosphorylation by CK1 is crucial for the subcellular localization of REV-ERBs, influencing Bmal1 transcription.

• Circadian rhythms in mammals rely on the expression of clock genes regulated by feedback loops.
• Bmal1, a key clock gene, shows a nocturnal transcriptional pattern in the mouse brain's suprachiasmatic nucleus.
• RORs induce and REV-ERBs repress the transcription of Bmal1.
• REV-ERBα and REV-ERBβ share highly homologous N-terminal regions that affect their phosphorylation and localization.
• Three serine-rich clusters in the N-terminal region of REV-ERBβ are necessary for its phosphorylation and cytoplasmic localization.
• REV-ERB phosphorylation by CK1 is associated with the timing of Bmal1 transcriptional regulation.

Simplified