Polysaccharides from Phellinus linteus attenuate type 2 diabetes mellitus in rats via modulation of gut microbiota and bile acid metabolism

Feb 10, 2024International journal of biological macromolecules

Phellinus linteus polysaccharides may reduce type 2 diabetes in rats by changing gut bacteria and bile acid processing

AI simplified

Circadian Biology on OpenScience ↗PubMed ↗DOI ↗

Abstract

Polysaccharides from Phellinus linteus (PLP) improved diabetes symptoms in a rat model of Type 2 diabetes mellitus (T2DM).

PLP increased the abundance of specific gut bacteria associated with the production of short-chain fatty acids (SCFAs) and bile acids (BAs).
PLP may regulate the composition of bile acids and enhance levels of short-chain fatty acids.
Analysis indicated that PLP stimulated the release of Glucagon-like peptide-1 (GLP-1), which is linked to insulin secretion.
The findings suggest that alterations in gut microbiota and bile acid metabolism by PLP could contribute to improved blood glucose levels in T2DM.

AI simplified

Type 2 diabetes mellitus (T2DM) is the most prevalent metabolic disorder. Polysaccharides from Phellinus linteus (PLP) have been found to have anti-diabetes effects, but the mechanism has not been elucidated. The purpose of this study was to investigate the mechanism of PLP on T2DM through the gut microbiota and bile acids metabolism. The T2DM rat model was induced by a high-fat high-carbohydrate (HFHC) diet and streptozocin (30 mg/kg). We found that PLP ameliorated diabetes symptoms. Besides, PLP intervention increased the abundance of g_Bacteroides, g_Parabacteroides, and g_Alistioes, which are associated with the biosynthesis of short-chain fatty acids (SCFAs) and bile acids (BAs) metabolism. Meanwhile, untargeted and targeted metabolomics indicated that PLP could regulate the composition of BAs and increase the levels of SCFAs. Real-time quantitative PCR (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA) were performed to analyze the expression levels of BAs metabolism enzymes in the liver. Finally, the results of correlation analysis and Glucagon-like peptide-1 (GLP-1) showed that PLP stimulated the release of GLP-1 by regulating SCFAs and BAs. In conclusion, this study demonstrated that PLP can regulate gut microbiota and BAs metabolism to promote GLP-1 secretion, thereby increasing insulin release, decreasing blood glucose and attenuating T2DM.

Full Text

Full text is available at the source.

View full text (XML) ↗View full text ↗

Polysaccharides from Phellinus linteus attenuate type 2 diabetes mellitus in rats via modulation of gut microbiota and bile acid metabolism

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief

Abstract

Full Text

Related papers

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief