Postprandial response and tissue distribution of the bile acid synthesis-related genes, cyp7a1, cyp8b1 and shp, in rainbow trout Oncorhynchus mykiss

Cloning of cyp7a1, cyp8b1, and shp genes from rainbow trout identified two isoforms each of these enzymes.

• Cyp7a1-1 and Cyp7a1-2 both encode proteins of 512 amino acids.
• Cyp7a1-1 is expressed primarily in the kidney, pyloric caecum, and mid-gut, with weak expression in the liver, eye, gill, and ovary.
• Cyp7a1-2 shows high expression in the liver, pyloric caecum, and mid-gut.
• Cyp8b1-1 and Cyp8b1-2 encode proteins of 512 and 509 amino acids, respectively, and are primarily expressed in the liver.
• SHP-1 and SHP-2 encode proteins of 288 and 290 amino acids, respectively, with strong expression of both in the liver; SHP-2 is also highly expressed in the gallbladder and digestive tract.
• Expression of cyp7a1-1/-2 and cyp8b1-1/-2 increases within 3 hours post feeding, while SHP-2 expression decreases before returning to initial levels at 24 hours.

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