The effect of pregnancy on the expression of uterine oxytocin, oestrogen and progesterone receptors during early pregnancy in the cow

Dec 17, 1998The Journal of endocrinology

How Early Pregnancy Changes Uterine Receptors for Oxytocin, Estrogen, and Progesterone in Cows

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Abstract

The release of the metabolite 13,14-dihydro-15-keto PGF2alpha (PGFM) after an oxytocin challenge was significantly higher in non-pregnant cows (187% ± 15%) compared with pregnant cows (131% ± 11%) (P < 0.01).

Oxytocin receptor (OTR) mRNA was found at low levels in the uterine luminal epithelium of some non-pregnant cows, but OTR protein was only detected in a few.
OTR mRNA and protein were undetectable in all pregnant cows, suggesting embryo presence suppresses OTR expression.
Estrogen receptor (ER) mRNA was present in both pregnant and non-pregnant cows, with no significant differences observed in ER expression between the groups.
Progesterone receptor (PR) mRNA showed variable expression in the caruncular stroma of both groups, but levels were generally lower in the dense caruncular-like stroma and glands.
The findings indicate that while the embryo suppresses OTR expression by day 16, it does not affect ER expression.

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The expression of oxytocin receptor (OTR) in the uterine endometrium plays an important role in the initiation of luteolysis. During early pregnancy, the conceptus secretes interferon tau (IFN|gt) which inhibits OTR up-regulation and luteolysis. In this study, uterine horn cross sections were collected on day 16 from 15 pregnant cows (PREG), 9 uninseminated controls and 5 inseminated cows with no embryo present. The latter two groups had similar results and were combined to form a single non-pregnant (NP) group. The animals were given an oxytocin challenge shortly before tissue collection to assess prostaglandin F2alpha (PGF2alpha) release through the measurement of the metabolite 13,14-dihydro-15-keto PGF2alpha (PGFM). The mRNAs for OTR, oestrogen receptor (ER) and progesterone receptor (PR) were localised by in situ hybridisation. The results were quantified by optical density (OD) measurements from autoradiographs using image analysis. OTR protein was measured by autoradiography with iodinated oxytocin antagonist and ER and PR protein was detected by immunocytochemistry. The release of PGFM after the oxytocin challenge was significantly higher in the 14 NP cows (187%+/-15%) compared with the PREG group (131%+/-11%) (P<0.01). Low concentrations of OTR mRNA were localised to the luminal epithelium (LE) in 6 out of the 14 NP cows, of which 2 also expressed OTR protein, while OTR mRNA and protein were undetectable in all the pregnant animals. These results indicated that the sampling time coincided with the onset of the luteolytic mechanism in the NP cows. On day 16 ER mRNA was detectable in both the LE and glands of both PREG and NP animals. There were no differences in either ER mRNA or protein between NP and PREG samples. PR mRNA was moderately expressed in the caruncular stroma, with lower levels in the dense caruncular-like stroma and glands. There were no differences between PREG and NP animals. The expression of PR mRNA and protein in the deep glands was variable between animals. These results suggested that, in cows, the presence of an embryo suppressed the expression of OTR, but had no effect on the expression of the transcriptionally regulated ER on day 16.

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The effect of pregnancy on the expression of uterine oxytocin, oestrogen and progesterone receptors during early pregnancy in the cow

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