Prime editing: improved CRISPR-based genome editing for plants

Prime editing enables precise installation of all possible 12 base-to-base conversions without inducing double-strand breaks.

• Prime editing can facilitate targeted insertions, deletions, and combinatorial modifications in genomes.
• The technology has been rapidly adopted in plant systems for functional genomics and trait improvement.
• Key advancements include innovations in protein engineering and improvements to the architecture of prime editing guide RNA.
• Modulating host DNA repair mechanisms may enhance editing efficiency and precision.
• The review discusses adaptations of prime editing systems for various crop species.

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