Prime editing in mammals: potential and real-world challenges

Prime editing can generate small-scale insertions, deletions, and substitutions in the genome with reduced risk of unwanted edits.

• This technology integrates a reverse transcriptase with a Cas9 nickase to facilitate targeted genome editing.
• Variability in editing outcomes has been reported across different studies using various methodologies and disease models.
• The review discusses how different approaches can influence the efficiency of prime editing.
• Reporter assays are highlighted as methods to detect editing events and select for edited cells.
• Downstream effects of prime editing are examined, along with potential reasons for differences observed among models.

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