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Abstract
Prime editing has been adapted for precise genome modifications in plants but has revealed limitations such as reduced efficiency and strong locus dependency.
- Key limitations of prime editing include low germline transmission and somatic chimerism.
- Diverse prime editing variants have emerged, leading to a fragmented landscape of performance.
- Optimization strategies are evaluated for their potential applications in monocots and eudicots.
- Emerging design principles aim to address persistent bottlenecks in prime editing.
- Future directions may involve AI-guided protein engineering and improved delivery strategies.
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