Journal of microbiological methodsJul 27, 2026

Fast and easy visual detection of Lawsonia intracellularis using a DNA-based test with CRISPR technology

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated Aug 3, 2026

Abstract

The RPA-CRISPR/Cas12a assay detects Lawsonia intracellularis in one hour with 100% concordance with qPCR results.

Lawsonia intracellularis is linked to porcine proliferative enteropathy, leading to diarrhea and poor growth in pigs.
Current detection methods lack the sensitivity and speed required for effective management of infections.
The new assay offers significant advantages in sensitivity and accuracy over existing techniques.
It shows no cross-reactivity with other common swine pathogens.
Results are easily interpreted visually under blue light.

Simplified

The swine industry faces a substantial economic threat from Lawsonia intracellularis (LI), the bacterium responsible for porcine proliferative enteropathy. Infection commonly presents with diarrhea and increased enterocyte proliferation in the ileum and colon, ultimately resulting in impaired growth performance in affected pigs. Effective management of porcine proliferative enteropathy requires timely and accurate diagnosis. The method provides significant advantages over existing detection techniques, offering enhanced sensitivity and high accuracy. The assay achieves detection within one hour, with no observed cross-reactivity against a panel of common swine pathogens. Results can be visually interpreted under blue light. Clinical validation using 123 samples demonstrated 100% concordance with qPCR results (14 positive and 109 negative). Characterized by simplicity, rapidity, and high sensitivity, the RPA-CRISPR/Cas12a method represents a novel solution for Lawsonia intracellularis detection.

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Fast and easy visual detection of Lawsonia intracellularis using a DNA-based test with CRISPR technology

Abstract

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