Virus genesJul 29, 2015

Proteins from two rice viruses in Vietnam may block the plant’s RNA silencing defense system

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated Mar 9, 2026

Abstract

Protein p6 of RRSV was confirmed to have mild silencing suppressor activity.

Rice grassy stunt virus (RGSV) and rice ragged stunt virus (RRSV) significantly reduce rice production in Vietnam.
An RNA interference (RNAi) strategy may provide a way to develop resistance to these viruses.
Identified RGSV genes include s2gp1, s5gp2, and s6gp1, while RRSV genes include s5gp1, s6gp1, s9gp1, and s10gp1.
Protein p6 of RRSV delays the systemic silencing signal, indicating its role as a mild silencing suppressor.
Two additional silencing suppressors with mild activity were identified: protein p2 of RGSV and protein p9 of RRSV.

Simplified

In Vietnam, the two main viruses that cause disease in rice are the Rice grassy stunt virus (RGSV) and the Rice ragged stunt virus (RRSV). Outbreaks of these two viruses have dramatically decreased rice production in Vietnam. Because natural resistance genes are unknown, an RNAi strategy may be an alternative method to develop resistance to RGSV and RRSV. However, this strategy will be efficient only if putative silencing suppressors encoded by the two viruses are neutralized. To identify these suppressors, we used the classical green fluorescent protein (GFP) agroinfiltration method in Nicotiana benthamiana. Then, we investigated the effects of viral candidate proteins on GFP expression and GFP siRNA accumulation and their interference with the short- or long-range signal of silencing. RGSV genes s2gp1, s5gp2, and s6gp1 and RRSV genes s5gp1, s6gp1, s9gp1, and s10gp1 were selected for viral silencing suppressor investigation according to their small molecular weight, the presence of cysteines, or the presence of a GW motif in related protein products. We confirmed that protein p6 of RRSV displays mild silencing suppressor activity and affects long-range silencing by delaying the systemic silencing signal. In addition, we identified two new silencing suppressors that displayed mild activity: p2 of RGSV and p9 of RRSV.

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Proteins from two rice viruses in Vietnam may block the plant’s RNA silencing defense system

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