The Plant journal : for cell and molecular biologySep 28, 2021

The TaCRK10 protein helps wheat seedlings resist stripe rust in heat by working with TaH2A.1

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated Mar 5, 2026

Abstract

The CRK gene TaCRK10 from wheat variety Xiaoyan 6 is associated with high-temperature seedling-plant resistance to stripe rust caused by the fungus Puccinia striiformis.

TaCRK10 expression increases following Pst inoculation and high temperature treatment.
Knockdown of TaCRK10 reduces wheat resistance to Pst at high temperatures but does not affect pathogen development or host responses at normal temperatures.
Overexpression of TaCRK10 in a susceptible wheat variety boosts resistance under normal temperatures, linked to increased reactive oxygen species and activation of defense-related genes.
TaCRK10 interacts with and phosphorylates the histone variant TaH2A.1, which positively influences wheat resistance to Pst.
TaCRK10 may act as a key sensor for Pst infection and high temperatures, regulating wheat resistance through nuclear processes.

Simplified

Plants sense various pathogens and activate immunity responses through receptor-like kinases (RLKs). Cysteine-rich receptor-like kinases (CRKs) are involved in massive transduction pathways upon perception of a pathogen. However, the roles of CRKs in response to stripe rust are unclear. In the present study, we identified a CRK gene (designated TaCRK10) from wheat variety Xiaoyan 6 (XY6) that harbors high-temperature seedling-plant (HTSP) resistance to stripe rust caused by fungal pathogen Puccinia striiformis f. sp. tritici (Pst). The expression level of TaCRK10 was induced by Pst inoculation and high temperature treatment. Knockdown of TaCRK10 by virus-induced gene silencing resulted in attenuated wheat HTSP resistance to Pst, whereas there is no effect on Pst development and host responses under normal temperatures. Notably, overexpression of TaCRK10 in susceptible variety Fielder provided resistance only under normal temperatures at 14 days with reactive oxygen species accumulation and defense-related gene expression of the salicylic acid pathway. Moreover, TaCRK10 physically interacted with and phosphorylated a histone variant TaH2A.1, which belongs to the H2A.W group. Silencing of TaH2A.1 suppressed wheat resistance to Pst, indicating that TaH2A.1 plays a positive role in wheat resistance to Pst. Thus, TaCRK10 serves as an important sensor of Pst infection and high temperatures, and it activates wheat resistance to Pst through regulating nuclear processes. This knowledge helps elucidate the molecular mechanism of wheat HTSP resistance to Pst and promotes efforts in developing wheat varieties with resistance to stripe rust.

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The TaCRK10 protein helps wheat seedlings resist stripe rust in heat by working with TaH2A.1

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