Self-oxygenating, autonomous self-healing dual-physical crosslinked PVA/chitosan/hydrolysed collagen hydrogels for advanced wound management

Aug 19, 2025Journal of materials chemistry. B

Self-oxygenating, self-healing hydrogels made from PVA, chitosan, and collagen for improved wound care

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Abstract

The hydrogel achieved over 95% wound closure within three days in a scratch wound assay.

Dissolved oxygen levels significantly increased with the addition of calcium peroxide, with 20 mg per 10 g gel providing sustained release over seven days.
The hydrogel demonstrated excellent viscoelasticity and mechanical strength, maintaining elasticity without rigidity.
Optimization of borax content and freeze-thaw cycles resulted in a hydrogel with self-healing properties and enhanced mechanical characteristics.
The combination of calcium peroxide and chitosan achieved 100% inhibition of Staphylococcus aureus, indicating strong antimicrobial effects.
Biocompatibility tests showed excellent cytocompatibility with L929 fibroblasts, with cell viability reaching 107%.

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Impaired wound healing in diabetic patients is a significant healthcare concern, often leading to microbial infections and delayed recovery due to the absence of an optimal healing environment. An ideal wound dressing should provide self-oxygenation, self-healing properties, mechanical strength, antimicrobial activity, and biocompatibility, which are crucial factors for cell survival and tissue regeneration. This study developed a dual physically crosslinked hydrogel composed of polyvinyl alcohol (PVA), chitosan (CS), and collagen peptide (Col), incorporating polycaprolactone (PCL)-coated calcium peroxide (CPO) particles. The effects of CPO, borax, and freeze-thaw (FT) cycles on the hydrogel's physicochemical properties and biocompatibility were analyzed. CPO significantly increased dissolved oxygen levels, with 20 mg CPO per 10 g gel providing sustained oxygen release over seven days, while higher CPO concentrations led to an initial burst release. The hydrogel exhibited excellent viscoelasticity and mechanical strength, maintaining elasticity without becoming rigid. Optimizing borax content and FT cycles resulted in a self-healing hydrogel with enhanced mechanical properties. The combination of CPO and CS achieved 100% inhibition of, demonstrating strong synergistic antimicrobial effects.biocompatibility was assessed using an MTT assay on hydrogel extracts, revealing excellent cytocompatibility with L929 fibroblasts, with cell viability reaching 107%. Additionally, Calcein-AM/PI live/dead staining of cells cultured directly on the hydrogels confirmed sustained cell adhesion and healthy spindle-like morphology. A scratch wound assay further demonstrated enhanced fibroblast migration, achieving over 95% wound closure within three days. These findings highlight the potential of this composite hydrogel as a multifunctional wound dressing for accelerating wound healing. Staphylococcus aureus In vitro

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Self-oxygenating, autonomous self-healing dual-physical crosslinked PVA/chitosan/hydrolysed collagen hydrogels for advanced wound management

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