Semi-Interpenetrating Hydrogel with Long-Term Intrinsic Antibacterial Properties Promotes Healing of Infected Wounds In Vivo

Oct 10, 2024ACS applied bio materials

Long-Lasting Antibacterial Hydrogel Helps Heal Infected Wounds in Living Tissue

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

The PVA/PPG-HBPL hydrogel dressing killed 99.9% of methicillin-resistant Staphylococcus aureus cultured on its surface without observable cytotoxicity.

The hydrogel demonstrated long-term antibacterial performance due to decreased release of antibacterial agents during its fabrication.
It significantly shortened the healing process by 2 orders of magnitude in MRSA-infected full-thickness skin wounds in rats.
The dressing was effective in reducing inflammatory cell infiltration and promoting new blood vessel formation and collagen deposition.
Mechanical properties were enhanced through hydrogen bonding and semi-interpenetrating networks.

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Bacterial infections significantly deteriorate the process of wound healing. The wound dressings loaded with antimicrobials are widely used to reduce bacterial infections. However, release-based sterilization may increase the risk of drug resistance of bacteria and complicate translation. Thus, the development of long-term intrinsic antibacterial wound dressings is highly desirable. In this study, an intrinsic antibacterial hydrogel (PVA/PPG-HBPL) consisting of poly(vinyl alcohol) (PVA), poly(polyethylene glycol methyl ether methacrylate--glycidyl methacrylate) (PPG), and hyperbranched poly-l-lysine (HBPL) was designed and fabricated. The mechanical properties of the PVA/PPG-HBPL hydrogel were enhanced by hydrogen bonding and semi-interpenetrating networks. It also possessed a favorable ability to absorb the wound exudates. The release of antibacterial HBPL was significantly decreased by the methods of cyclic freeze-thawing and covalent cross-linking during hydrogel fabrication, enabling the PVA/PPG-HBPL hydrogel with intrinsic and long-term antibacterial performance. The PVA/PPG-HBPL hydrogel dressing killed 99.9% of methicillin-resistant(MRSA) cultured on its surface without observable cytotoxicity. It observably shortened the healing process by 2 orders of magnitude ofcolonies compared with the control in the MRSA-infected full-thickness skin wound of ratseven after being soaked in phosphate-buffered saline (PBS) for 21 days (PBS was changed every 3 days). The antibacterial hydrogels could kill wound bacteria in a timely manner, significantly reduce inflammatory cell infiltration, and promote neovascularization and collagen deposition. co Staphylococcus aureus in vitro MRSA in vivo

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Semi-Interpenetrating Hydrogel with Long-Term Intrinsic Antibacterial Properties Promotes Healing of Infected Wounds In Vivo

Abstract

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