Nature reviews. Molecular cell biology

SenNet guidelines for identifying aging cells in various tissues

Updated

Abstract

Cellular senescence may play both beneficial and detrimental roles in various biological processes across humans, rodents, and other species.

  • Cellular senescence is associated with a permanent stop in cell division and has been linked to different biological processes.
  • Existing knowledge largely stems from tissue culture studies, where cells are forced into senescence.
  • Senescent cells are rare in tissues, making them challenging to identify and study.
  • Fully differentiated, non-dividing cells can also exhibit features of senescence.
  • Recommendations for detecting senescent cells in tissues are based on a review of senescence markers found in 14 tissues in mice and humans.
  • Recent advances include the identification of molecular signatures and morphological characteristics of senescent cells.

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Funding

Competing interests

Competing interests: The authors declare the following competing interests: J.C. is a founder and shareholder of Unity Biotechnology, which develops senolytic drugs. M.J.S., C.M.C. and Mayo Clinic have intellectual property related to this research. Research in the M.J.S. and D.J.B. laboratory is reviewed by the Mayo Clinic Conflict of Interest Review Board and conducted in compliance with Mayo Clinic Conflict of Interest policies. D.J.B. has a potential financial interest related to this research; he is a co-inventor on patents held by Mayo Clinic and patent applications licensed to or filed by Unity Biotechnology and a Unity Biotechnology shareholder. N. Slavov is a founding director and CEO of Parallel Squared Technology Institute, which is a non-profit research institute.
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