Single-cell gene activity shows disrupted daily rhythms between cells in the Alzheimer's-affected human brain

ORPHEUS suggests that excitatory neurons in Alzheimer's disease dementia have marked loss of cellular circadian synchrony.

This was a single-cell time-course analytical-method study validating ORPHEUS in silico and in mouse SCN data, then applying it to mouse liver and human brain datasets.

The Alzheimer's finding comes from applying a computational variance method to existing single-cell data, so it depends on model assumptions and does not show functional consequences directly.