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Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a
How CRISPR-Cas12a processes guide RNA and targets DNA based on initial matching
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Abstract
Crystal structures of Cas12a reveal distinct mechanisms for guide RNA processing and target DNA cleavage.
- Cas12a exhibits two nuclease activities: processing its guide RNAs and cleaving target DNA.
- The structures show how guide RNA is processed and how its seed sequence is prepared for binding.
- An R-loop complex structure illustrates the mechanism of guide-target hybridization.
- The findings suggest a single active site mechanism for double-stranded DNA cleavage.
- Insights gained may enhance understanding of Cas12a enzymes for genome editing applications.
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