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Abstract
The average particle size of the survivin mRNA lipid nanoparticles was 305 ± 68 nm.
- Survivin mRNA lipid nanoparticles (LNPs) demonstrated a polydispersity index of 0.11 and encapsulation efficiency of 65 ± 3.9%.
- Incubation of survivin mRNA-LNPs with mouse-derived bone marrow dendritic cells resulted in the activation of tumor-associated antigen specific T-cells.
- Significant reduction in cancer cell viability (P < 0.05) was observed in co-cultures of splenocytes and cheek cells from C57BL/6 mice treated with survivin mRNA-LNPs and a CTLA-4 inhibitor.
- The reduction in cancer cell viability may be associated with the upregulation of MHCI and MHCII, as well as an increase in CD8+ and CD4+ T-lymphocytes and a decrease in T-regulatory cells.
- These findings suggest the potential for further development of survivin mRNA-LNPs combined with a CTLA-4 inhibitor for oral cancer immunotherapy.
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mRNA Technology