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Rapid, sensitive, and visual detection of swine Japanese encephalitis virus with a one-pot RPA-CRISPR/EsCas13d-based dual readout portable platform
Fast and clear portable test for detecting swine Japanese encephalitis virus using a combined CRISPR-based method
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Abstract
The novel one-pot detection method can identify as few as two copies of Japanese encephalitis virus nucleic acid within 1 hour.
- The method combines recombinant enzyme polymerase isothermal amplification with CRISPR/EsCas13d targeting.
- Visual fluorescence and lateral flow assay results are produced for easy interpretation.
- The platform demonstrated 100% concordance with real-time PCR results when validated against clinical samples.
- It shows no cross-reactivity with other pathogens, indicating high specificity.
- This approach may serve as an effective point-of-care testing solution for Japanese encephalitis detection in remote settings.
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